C-peptide chemiluminescence immunodetection kit, preparation method and detection method therefor

• Main Authors: HAN MEIYU, GAO WEI, SUN CHENGYAN, HE HAOHUI, WANG LIYING
• Format: Patent
• Language: Chinese; English
• Published: 18.01.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a C-peptide chemiluminescence immunodetection kit, a preparation method and a detection method therefor, belongs to the field of the immunoassay, and solves the problems of lowsensitivity, long reaction time, and high cost of an existing C-P detection method. The kit comprises magnetic particles coated by the streptavidin; the C peptide antibody labeled by the biotin; theC peptide antibody labeled by the acridinium ester; the wash buffer; the chemoluminescence pre-trigger solution; a calibrator; and a quality control product. The detection method is as follows: the C-peptide antigen in the sample is immunologically reacted with the C-peptide antibody labeled by the biotin and the C peptide antibody labeled by the acridinium ester to form a biotin-C peptide antibody-C peptide antigen-C peptide antibody-acridinium ester solvent; the magnetic particles coated by the streptavidin are combined with the biotin to form a magnetic bead-streptavidin-biotin-C peptide antibody-C peptide antigen-C