Swine Mycoplasmal pneumonia live vaccine mucosal immune adjuvant, preparation method and application thereof

• Main Authors: XIONG QIYAN, GAN YUAN, ZHANG ZHENZHEN, WANG JIA, ZHANG LEI, WEI YANNA, SHAO GUOQING, FENG ZHIXIN, BAI YUN
• Format: Patent
• Language: Chinese; English
• Published: 15.01.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a swine Mycoplasmal pneumonia live vaccine mucosal immune adjuvant, a preparation method and application thereof, which relate to the field of veterinary vaccines. The mucosal adjuvant is a solution of a recombinant protein CTB-P97R1 containing fusion expression of cholera toxin B subunit and Mycoplasma hyopneumoniae P97R1. The invention also provides the preparation methodof the mucosal adjuvant, wherein preparing 10 mg/ml recombinant protein CTB-P97R1 mother liquor, then diluting the mother liquor with a solvent. The invention also provides the application of the mucosal immune adjuvant in preparing a live vaccine of Mycoplasmal pneumonia . The mucosal adjuvant of that invention is an aqueous compound adjuvant, The preparation method is simple, the adjuvant property is stable, the mycoplasma hyopneumoniae activity is not damaged, and the mixture is compatible with aerosol protectant, and the mixture is still harmless to mycoplasma hyopneumoniae activity, so itis suitable for aerosol i