Fluorescence in situ hybridization sequencing detection method for CYP gene single nucleotide polymorphism

• Main Authors: WANG HEYAO, SUN MEINA
• Format: Patent
• Language: Chinese; English
• Published: 11.01.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a fluorescence in situ hybridization sequencing detection method for CYP gene single nucleotide polymorphism. The method comprises extracting DNA of a sample to be tested, taking DNA as a template for single-strand derivatization, simultaneously adding different fluorescent labeled first sequencing probes and second sequencing probes to hybridize with the single-stranded derivative, and finally interpreting the hybridization result. The method has the advantages of high accuracy, good stability, fast, safety and easy automation, and can complete accurate typing of single nucleotide polymorphisms in a single strand derivatization and hybridization reaction cycle, thereby understanding the genotypes of subjects, and providing guidance for preventing diseases caused byrisk factors and clinically individualized medication. 本发明公开了种CYP基因单核苷酸多态性的荧光原位杂交测序检测方法，所述方法包括提取待测样本的DNA，并以DNA为模板进行单链化衍生，然后同时加入不同荧光标记的第测序探针和第二测序探针与单链化衍生物进行杂交，最后对杂交结果进行判读。所述方法精准性高，稳定性好，快速，安全，易自动化操作，可在个单链化衍生和杂交反应循环中完成对单核苷酸多态性的