CYP2D6 gene mutation detection primer and kit

• Main Authors: TANG JIA, ZENG QINLONG, HUANG HUI, XU JINMEI
• Format: Patent
• Language: Chinese; English
• Published: 11.01.2019
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a CYP2D6 gene mutation detection primer, comprising an upstream primer as shown in SEQ ID No. 1, a downstream primer as shown in SEQ ID No. 2 and a probe as shown in SEQ ID No.3. The invention also discloses the use of the detection primer in preparing a CYP2D6 gene mutation detection reagent and a CYP2D6 gene mutation detection reagent kit. The detection primer provided by the invention can realize the simultaneous detection of wild type of CYP2D6*10 (rs1065852 C100T) site in the same tube PCR reaction, heterozygous mutation and homozygous mutation, and has the advantages of simple operation, high sensitivity, high specificity and low cost. 本发明公开了种CYP2D6基因突变检测引物，包括如SEQ ID No.1所示的上游引物、SEQ ID No.2所示的下游引物和SEQ ID No.3所示的探针。本发明还公开了所述检测引物在制备CYP2D6基因突变检测试剂中的用途及种CYP2D6基因突变检测试剂盒。本发明提供的检测引物可实现在同管PCR反应中同时检测CYP2D6*10(rs1065852 C100T)位点的野生型、杂合突变及纯合突变，具有操作简单、灵敏度高、特异性高和成本低等优点。