Kit for measuring anti-histone antibody IgG and detection method
The invention relates to a kit for measuring an anti-histone antibody IgG. The kit is characterized by comprising a first reagent and a second reagent, the first reagent comprises a mixed antigen coupled with biotin, and the mixed antigen comprises a histone antigen, H1 fragments and H2A-H2B composi...
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Main Authors | , , , , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
04.01.2019
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Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to a kit for measuring an anti-histone antibody IgG. The kit is characterized by comprising a first reagent and a second reagent, the first reagent comprises a mixed antigen coupled with biotin, and the mixed antigen comprises a histone antigen, H1 fragments and H2A-H2B composite fragments; the second reagent comprises an antihuman IgG antibody coupled with alkaline phosphatase. The kit and a detection method have the advantages that the stability is good, the sensitivity is high and the repeatability is good; meanwhile, the detection time is short, a test is completed within 50 min, the operation is simple and convenient, and full-automatic detection is truly achieved. Besides, the kit reaches a high positive detection rate for SLE patients.
本发明涉及种测定抗组蛋白抗体IgG的试剂盒,其特征在于:所述的试剂盒包括第试剂和第二试剂,所述的第试剂包括偶联有生物素的混合抗原,所述的混合抗原包括组蛋白抗原、H1片段以及H2A-H2B复合片段;所述的第二试剂包括偶联有碱性磷酸酶的抗人IgG抗体。本发明的试剂盒及检测方法具有稳定性好,灵敏度高,重复性好等优点,同时检测时间短,完成个测试所需的总时间在50分钟以内,而且操作简单方便,真正实现了检测全自动化,另外,本发明的试剂盒SLE患者的阳性检出率高。 |
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Bibliography: | Application Number: CN201810921543 |