Kit for measuring anti-histone antibody IgG and detection method

• Main Authors: LIU LE, ZHAO TING, ZHI XIAOLE, LI JINGWEN, CUI LIGE, XU LE, YU LU, LI YUMING, KONG QIAOYUN, YANG SUQING
• Format: Patent
• Language: Chinese; English
• Published: 04.01.2019
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a kit for measuring an anti-histone antibody IgG. The kit is characterized by comprising a first reagent and a second reagent, the first reagent comprises a mixed antigen coupled with biotin, and the mixed antigen comprises a histone antigen, H1 fragments and H2A-H2B composite fragments; the second reagent comprises an antihuman IgG antibody coupled with alkaline phosphatase. The kit and a detection method have the advantages that the stability is good, the sensitivity is high and the repeatability is good; meanwhile, the detection time is short, a test is completed within 50 min, the operation is simple and convenient, and full-automatic detection is truly achieved. Besides, the kit reaches a high positive detection rate for SLE patients. 本发明涉及种测定抗组蛋白抗体IgG的试剂盒，其特征在于：所述的试剂盒包括第试剂和第二试剂，所述的第试剂包括偶联有生物素的混合抗原，所述的混合抗原包括组蛋白抗原、H1片段以及H2A-H2B复合片段；所述的第二试剂包括偶联有碱性磷酸酶的抗人IgG抗体。本发明的试剂盒及检测方法具有稳定性好，灵敏度高，重复性好等优点，同时检测时间短，完成个测试所需的总时间在50分钟以内，而且操作简单方便，真正实现了检测全自动化，另外，本发明的试剂盒SLE患者的阳性检出率高。