Fluorescent quantitative PCR method for detecting potato leaf roll virus

• Main Authors: YE XINYOU, MAO LUTIAN, HOU HONGYIN, CHEN ZHAOGUI, XING SHUQI, HUANG YAN
• Format: Patent
• Language: Chinese; English
• Published: 28.12.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of biological detection, in particular to a fluorescent quantitative PCR method for detecting a potato leaf virus. The fluorescent quantitative PCR methodcomprises the following steps that 1, RNA extraction is performed, wherein the total RNA of potatoes is extracted; 2, reverse transcription is performed, the extracted total RNA is reversely transcribed into cDNA; 3, primer design is performed, wherein two pairs of primers for amplification of an internal reference gene and a PLRV gene are respectively designed; 4, fluorescent quantitative PCR isperformed, the reversely transcribed cDNA and the designed primers are prepared into a PCR reaction system, and real-time fluorescent quantitative PCR reaction is performed. By adopting the detectionmethod, it can be detected at the molecular level that the potato leaf virus exists in potato plants, the virus gene expression situation can be quantitatively analyzed, and the fluorescent quantitative PCR method has the advan