Method for screening variable zones of specific antibodies

The invention discloses a method for screening single-chain antibodies by utilizing a bacterial surface display system. The method comprises the following steps that A, a gene encoding library consisting of different single-chain antibody encoding genes to be detected is established, wherein primer...

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Bibliographic Details
Main Authors REN GUIPING, LI DESHAN, BAI YIN, GUO XIAOCHEN
Format Patent
LanguageChinese
English
Published 28.12.2018
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a method for screening single-chain antibodies by utilizing a bacterial surface display system. The method comprises the following steps that A, a gene encoding library consisting of different single-chain antibody encoding genes to be detected is established, wherein primer pairs for amplifying antibodies VH and VL are both the same kind of degenerate primer pairs, and gene joints for connecting the VH and VL are the same kind of CH1; B, gene encoding sequences of target single-chain antibodies specific to target antigens are acquired, wherein the target antigens and single-chain antibodies to be detected are co-expressed, the antigens are marked with tag protein Flag, and after recombinant bacteria carrying the target single-chain antibodies are obtained through screening by means of fluorescent marker anti-tag protein antibodies and a flow cell sorting method, and the target single-chain antibodies are rescued through a method for reconverting host bacteria by extracting plasmids. B
Bibliography:Application Number: CN20181741545