Time resolution fluorescent immunochromatographic test strip for detecting pendimethalin, and preparation method and application thereof

• Main Authors: CUI HUAPENG, CHEN LI, LIU HUIMIN, FAN ZIYAN, XIE FUWEI, ZHANG XIAOBING, YU JINGJING, TANG GANGLING, WANG XIAOYU, FAN MEIJUAN
• Format: Patent
• Language: Chinese; English
• Published: 21.12.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting pendimethalin, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate, a sample absorbent pad, a combination absorbent pad, a nitrocellulose membrane and a water absorbent pad. A pendimethalin monoclonal antibody labelled by fluorescent microspheres is embedded into the combination absorbent pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A pendimethalin hapten-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the quality control area. A pendimethalin hapten is obtained in the modes that N-(1-ethyl propyl ether)-3,4-methyl toluidine and 4-bromo-butyric acid ethyl ester react to generate hydrocarbylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, then the hydrocarbylation N-(1-ethyl propylether)-3,4-methyl toluid