Time resolution fluorescent immunochromatographic test strip for detecting carbendazim, and preparation method and application thereof

• Main Authors: ZHAO LE, YAN QUANPING, CUI HUAPENG, PAN LINING, CHEN LI, LIU HUIMIN, WANG SHENG, FAN ZIYAN, TANG GANGLING, FAN MEIJUAN
• Format: Patent
• Language: Chinese; English
• Published: 21.12.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a time resolution fluorescent immunochromatographic test strip for detecting carbendazim, and a preparation method and application thereof. The time resolution fluorescent immunochromatographic test strip comprises a bottom plate and further comprises a sample absorbent pad, a combination releasing pad, a nitrocellulose membrane and a water absorbent pad which are lapped and pasted on the bottom plate in sequence. A carbendazim monoclonal antibody labelled by fluorescent microspheres is embedded into the combination releasing pad, and a detecting area and a quality control area are fixed to the nitrocellulose membrane. A carbendazim hapten-carrier protein conjugate is sprayed in the detecting area, and an anti-sheep and anti-mouse antibody is sprayed in the qualitycontrol area. A carbendazim hapten is obtained in the mode of reacting of 2-aminobenzimidazole with butanedioic anhydride. The invention further provides the preparation method of the test strip and amethod for detecting the