HPTLC-生物显影筛检油脂合成酚类抗氧化剂的方法

• Format: Patent
• Language: Chinese
• Published: 09.10.2020
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

本发明涉及HPTLC-生物显影筛检油脂合成酚类抗氧化剂的方法，属于食品检测技术领域。根据酚类抗氧化剂可以通过清除自由基反应改变反应体系颜色的特点，建立了一种基于抗氧化活性显色的分析方法，对待测样品进行衍生化反应，并利用HPTLC作为开放的色谱分离载体，再使用薄层色谱扫描仪进行扫描定量。本发明的方法检测周期短，准确性好，检测结果基本与HPLC检测结果一致。 The invention relates to a HPTLC-method for screening the synthesized phenolic antioxidants in oil and fat by bioautography, belonging to the field of food testing technology. According to the property that phenolic antioxidants changes color by removing the free radical groups from other reactants in a reaction system, the analytical method is established based on antioxidant color development. The testing sample first undergoes a derivative reaction, then using HPTLC as the open chromatographic separation carrier the sample is quantitatively scanned with a thin-layer chromatography scanner.The method of the invention had the advantage of short testing time and satisfactory accuracy. The testing result is generally consistent with that of HPLC.