Method for extracting apolipoprotein B from serum and apolipoprotein B

• Main Author: KE TUO
• Format: Patent
• Language: Chinese; English
• Published: 21.12.2018
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

The invention provides a method for extracting apolipoprotein B from serum and the apolipoprotein B and relates to the technical field of biochemistry. The method for extracting the apolipoprotein B from serum comprises the steps as follows: a crude sample, obtained after pretreatment of serum, of apolipoprotein B is subjected to hydrophobic chromatography, and the apolipoprotein B is obtained after elution. The apolipoprotein B is extracted with the hydrophobic chromatography method, the purification efficiency of the apolipoprotein B is significantly improved, the problem of low purity of protein extracted with a chemical precipitation method in the prior art is relieved, the problem of high cost of a supercentrifugation method is solved, and the concentration and purity of the extractedapolipoprotein B can be greatly improved. Besides, the method is simple to operate, low in cost and easy to promote and apply and has low requirement for equipment and technicians. Moreover, the method also has the advantage