Method for producing vitamin B12 by using Pseudomonas denitrificans based on pH value control

• Main Authors: ZHANG GUANGYONG, YANG HUI, LIU YANMAO, YU JU
• Format: Patent
• Language: Chinese; English
• Published: 30.11.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of biological fermentation, and discloses a method for producing vitamin B12 by using Pseudomonas aeruginosa based on pH value control. The method for producing the vitamin B12 by using the Pseudomonas aeruginosa based on the pH value control comprises the steps that a production strain of vitamin B12, namely the Pseudomonas aeruginosa is inoculated into a fermentation medium, and fermentation is carried out at a temperature of 25-30 DEG C; the composition of the fermentation medium comprises 70-85g/L of cane molasses, 90-110g/L of yeast extract, 0.05-0.2 g/L of 5,6-dimethylbenzimidazole, 0.1-1g/L of glycerophosphoric acid, 0.05-2g/L of cobalt chloride, 0.05-0.2g/L of urea, 10-30g/L of betaine, 0.05-0.2 g/L of arginine, 0.01-0.5g/L of thiamine and the like; the pH value of fermentation broth is controlled at 7 by the cane molasses and the urea; and the purified vitamin B12 is separated from the fermentation broth. The yield of the vitamin B12 is improved by control