Recombinant bovine long-acting interferon as well as fusion protein preparing long-acting interferon as well as preparation method of fusion protein

• Main Authors: BAO KEBING, GAO YAOHUI, XU MUZHEN, SHAN XUEQIN, XU WENJUN, ZHAO YU, XIA BINGBING, FAN YUFANG
• Format: Patent
• Language: Chinese; English
• Published: 23.11.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a recombinant bovine long-acting interferon as well as a fusion protein preparing the long-acting interferon as well as a preparation method of the fusion protein. The fusion protein is formed by connecting bovine interleukin 2 and bovine interferon alpha by virtue of flexible linker, after the fusion protein is mixed with a freeze drying protection agent, the mixture is freeze dried, thus the recombinant bovine long-acting interferon is obtained. By adopting the recombinant bovine long-acting interferon, the half-life period of the bovine interferon can be significantly prolonged and can be increased by 10 times or more compared with the half-life period of the ordinary bovine interferon, a broad-spectrum antivirus effect can be achieved, and the immune response ofthe cattle can be improved. 本发明公开了种重组牛长效干扰素及制备此长效干扰素的融合蛋白及其制备方法，所述融合蛋白由牛白细胞介素2与牛干扰素α经柔性linker连接而形成，融合蛋白与冻干保护剂混配后胡静冷冻干燥即可得到重组牛长效干扰素。所述重组牛长效干扰素可显著提高牛干扰素的半衰期，较普通牛干扰素的半衰期提高10倍以上，并具有广谱抗病毒作用并能提高牛自身的免疫应答。