Synthesis and application of fluorescent probe with specific recognition on GC33-3-1 antibody

• Main Authors: XU JIANHUA, WANG JIN, KE FANG, WU XIAOWEN, CHEN YAN, LIN QIAOFA, TU SHUQING
• Format: Patent
• Language: Chinese; English
• Published: 23.11.2018
• Subjects: ADHESIVES; CHEMISTRY; DYES; HETEROCYCLIC COMPOUNDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FORELSEWHERE; MEASURING; METALLURGY; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; NATURAL RESINS; ORGANIC CHEMISTRY; PAINTS; PHYSICS; POLISHES; TESTING

The invention discloses synthesis and application of a fluorescent probe with specific recognition on a GC33-3-1 antibody. A synthesis route of the probe is simple, reaction conditions are moderate and post-treatment is simple and convenient. After the fluorescent probe is labeled with human serum albumin (HSA), the fluorescence intensity is greatly enhanced; after the fluorescent probe is labeledwith the HSA, the fluorescent probe is used for recognizing the GC33-3-1 antibody. Compared with other methods for detecting the antibody, the method has the advantages of simplicity in operation, sensitive response, selectivity and the like, and has a relatively good application prospect in the field of biology; a formula is shown in the description. 本发明公开种针对GC33-3-1抗体具有特异性识别的荧光探针的合成以及应用。该探针合成路线简单，反应条件温和，后处理简单方便。该荧光探针标记人血清蛋白（HSA）后荧光强度大大增强，荧光探针标记HSA后再将其用于识别GC33-3-1抗体。与其他检测抗体的方法相比，该方法具有操作简便，响应灵敏，以及具有选择性等优点，在生物领域有较好的应用前景；。