Detection reagent combination and detection method for aquatic product additives

The invention discloses a detection reagent combination and a detection method for aquatic product additives. An aqueous solution of acetonitrile containing nitrofuran metabolites is used as a reference substance; a methanol mixture containing a methanol solution of 2-nitrobenzaldehyde and an aqueou...

Full description

Saved in:
Bibliographic Details
Main Authors LI YINGFEI, WU SICHANG
Format Patent
LanguageChinese
English
Published 20.11.2018
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online AccessGet full text

Cover

More Information
Summary:The invention discloses a detection reagent combination and a detection method for aquatic product additives. An aqueous solution of acetonitrile containing nitrofuran metabolites is used as a reference substance; a methanol mixture containing a methanol solution of 2-nitrobenzaldehyde and an aqueous solution of hydrochloric acid is used as a derivatizing agent; and a liquid chromatography-tandemmass spectrometry method is used for detection. The detection reagent combination provided by the invention can simultaneously analyze additives in aquatic products, and has the widest detection range; a scheme of simultaneous derivatization of protein precipitates is adopted in sample treatment to directly derivatize, purify and enrich the nitrofuran metabolites in biological samples and shortenthe derivatization time of a traditional method from 16 h to 10-20 min; and the detection method using the reagent combination is simple, accurate, rapid, stable, free from enzymatic conversion and other complicated steps, fre
Bibliography:Application Number: CN20181642406