Porcine albumin-interferon alpha-interleukin 2 fusion protein, preparation method as well as coding gene thereof, and porcine pegylated interferon

• Main Authors: BAO KEBING, GAO YAOHUI, XU MUZHEN, FU CHAO, ZHOU WEI, XU WENJUN, YANG JIANWEI, FAN YUFANG
• Format: Patent
• Language: Chinese; English
• Published: 20.11.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a porcine albumin-interferon alpha-interleukin 2 fusion protein, a preparation method as well as a coding gene thereof, and porcine pegylated interferon. Porcine albumin, porcine interferon alpha and porcine interleukin 2 are connected through a flexible linker to obtain the porcine albumin-interferon alpha-interleukin 2 fusion protein, the coding gene of the fusion proteinis designed to be optimized, and the recombinant porcine pegylated interferon is prepared finally; the porcine pegylated interferon can significantly improve a half-life period of porcine interferon,which is 27 times or more than 27 times of the half-life period of ordinary porcine interferon, has a broad-spectrum antiviral effect, and can improve the immune response of pigs themselves. 本发明公开了猪白蛋白-干扰素α-白介素2融合蛋白、制备方法及其编码基因、种猪长效干扰素，将猪白蛋白、猪干扰素α和猪白介素2通过柔性linker连接，得到猪白蛋白-干扰素α-白介素2融合蛋白，设计优化其编码基因，最终制备得到重组猪长效干扰素，可显著提高猪干扰素的半衰期，较普通猪干扰素的半衰期提高27倍以上，并具有广谱抗病毒作用并能提高猪自身的免疫应答。