Fusion protein comprising chicken albumin and chicken interferon gamma, preparation method of fusion protein, and recombination chicken long-acting interferon gamma

• Main Authors: JIANG MINZHI, BAO KEBING, GAO YAOHUI, SHAN XUEQIN, WANG YANAN, ZHOU WEI, YANG JIANWEI
• Format: Patent
• Language: Chinese; English
• Published: 20.11.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a fusion protein comprising a chicken albumin and a chicken interferon gamma, a preparation method of the fusion protein, and a recombination chicken long-acting interferon gamma. The fusion protein is formed by connecting the chicken albumin and the chicken interferon gamma through a flexible linker, and the recombination chicken long-acting interferon gamma is obtained byblending the fusion protein and a freezing and drying protecting agent, and freeze-drying. The recombination chicken long-acting interferon gamma obviously improves the half-life period of the chicken interferon, the half-life period is improved by 15 times or more in comparison with the common chicken interferon gamma, the recombination chicken long-acting interferon gamma has a broad-spectrum antiviral effect, and the immune response of the chicken is improved. 本发明公开了种由鸡白蛋白与鸡干扰素γ组成的融合蛋白及其制备方法和种重组鸡长效干扰素γ，所述融合蛋白由鸡白蛋白与鸡干扰素γ经柔性linker连接而形成，融合蛋白与冻干保护剂混配后经冷冻干燥即可得到重组鸡长效干扰素γ。所述重组鸡长效干扰素γ可显著提高鸡干扰素的半衰期，较普通鸡干扰素γ的半衰期提高15倍以上，并具有广