Raspberry tissue culture germination method

• Main Authors: CHU XIAOJIE, ZHA XIAOJUN, CHAI PIAOPIAO
• Format: Patent
• Language: Chinese; English
• Published: 20.11.2018
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention belongs to the technical field of plant culture and discloses a raspberry tissue culture germination method. The raspberry tissue culture germination method comprises the following steps: disinfecting raspberry seeds; inoculating the seeds into a WPM culture medium for culturing; performing subculture, and culturing by the WPM culture medium, wherein the WPM culture medium comprises0.56 g of calcium nitrate, 20 g/L of sucrose, 5 g/L of phytagel, 2.14 g/L of WPM powder, 1.5 mg/L of hormone ZT, and obtaining a plant with strong growth; taking the plant out of a conical flask, cleaning the root with clean water, and performing water culture in clean water for 15 days; performing earth culture with nutrient soil, thereby obtaining the final plant. According to the method disclosed by the invention, the germination rate is 86.7%; a plantlet with 2-3 leaves can be conveniently observed within about 10 days; in order to enable the plant to grow strong, subculture can be continuously performed by 2-3 mo