Preparation of double-labeled virus-like particles (VLPs) of PRRSV

The invention discloses a method for preparing and identifying double-labeled virus-like particles (VLPs) of PRRSV, and relates to the field of biological research technology; a pMD18T recombinant plasmid containing GP5, M and N protein coding genes ORF5, ORF6 and ORF7 is prepared as an amplificatio...

Full description

Saved in:
Bibliographic Details
Main Authors HOU QIAN, DAI JUNFEI, ZHANG JIE, OU YUNWEN, ZHANG YONGGUANG, WANG JUN, MA BING, LIU YONGSHENG, DING YAOZHONG, SUN YUEFENG, LI QIAN
Format Patent
LanguageChinese
English
Published 16.11.2018
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:The invention discloses a method for preparing and identifying double-labeled virus-like particles (VLPs) of PRRSV, and relates to the field of biological research technology; a pMD18T recombinant plasmid containing GP5, M and N protein coding genes ORF5, ORF6 and ORF7 is prepared as an amplification template, a Myc tag is introduced at the end of the ORF5, a His tag is introduced into the end ofthe ORF6, a recombinant plasmid is obtained by recombination to PpH and Pp10 promoters of a binary vector, the recombinant plasmid is transferred into DH10Bac E. coli to obtain a recombinant bacmid; and the ORF7 and a pFastBacHTB vector are recombined to obtain a recombinant bacmid. The two recombinant bacmids are inoculated into SF9 cells, and Myc-GP5, His-M and His-N proteins are successfully expressed and automatically assembled into the VLPs. The method lays a foundation for studying the function of the GP5 and M and N major structural proteins and developing VLP vaccines that can distinguish between infection and
Bibliography:Application Number: CN201810785057