Application of recombinant human lysozyme to preparation of medicine for resisting human immunodeficiency virus

• Main Authors: LYU QIUJUN, LI YANAN, LIANG JIACHAO, XIA FENGGENG, ZHENG JINLI, LI YUEQIAO, CHEN YUXING, YANG CHUANGQIN, ZHANG HUA, CHEN CONGWEI, DENG YUANNI, CHEN DAKAI
• Format: Patent
• Language: Chinese; English
• Published: 16.11.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a method for inhabiting the human immunodeficiency virus by human lysozyme and application of recombinant human lysozyme to preparation of a medicine for resisting human immunodeficiency virus (HIV). According to the method, the human lysozyme is in contact with cells infected with the HIV, and the human lysozyme is prepared through a gene recombination technology; the high-expression, high-purity and high-activity human lysozyme can be provided; the human lysozyme has relatively small cytotoxicity and an inhibition effect on syncytium formed by HIV-1IIIB infected C8166cells; the human lysozyme has remarkable anti-HIV activity and relatively high treatment indexes and can be used as an ideal option for preparing the medicine for diagnosing, preventing, treating andinhibiting human immunodeficiency virus. 本发明提供了种通过人溶菌酶抑制人类免疫缺陷病毒(HIV)的方法及其应用，所述方法通过使人溶菌酶接触感染HIV的细胞，所述的人溶菌酶通过基因重组技术制备而得，能够提供高表达、高纯度、高活性的人溶菌酶，且对细胞毒性较小，对HIV-1感染C8166细胞形成合胞体起抑制作用，具有显著的抗HIV活性和较高的治疗指数，可作为制备诊断、预防、治疗、抑制艾滋病药物的理想选择。