Compound enzyme for eggshell membrane hydrolysis, eggshell membrane hydrolysis method and eggshell membrane hydrolysis product and extracting method thereof

• Main Authors: TANG ZHANGYONG, CHEN YUN, ZHANG GE, CHEN CHENG
• Format: Patent
• Language: Chinese; English
• Published: 13.11.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the technical field of bioengineering and provides a compound enzyme for eggshell membrane hydrolysis. The compound enzyme comprises keratinase and alkaline protease in a massratio of 0.2-5:1, and the compound enzyme enables complete hydrolysis of eggshell membranes. An eggshell membrane hydrolysis method includes: mixing eggshell membrane dried powder with water, keepingthe temperature at 70-80 DEG C for 10-240min, cooling to 50-55 DEG C, and keeping the temperature to obtain eggshell membrane liquid; adjusting pH of the eggshell membrane liquid in a range of 8.0-8.5, adding the compound enzyme, and continuing heat preservation to obtain eggshell membrane hydrolysis mixed liquid. By adoption of the hydrolysis method, complete hydrolysis of the eggshell membranescan be realized. An extracting method of an eggshell membrane hydrolysis product includes the eggshell membrane hydrolysis method and includes steps of adjusting pH to 5.0-8.0, heating to 90-100DEG C, keeping the temperature f