SYSTEMS AND METHODS FOR ALLERGEN DETECTION

• Main Authors: YOUNG ADAM J, JACOBS ADAM, BABU BROWN RENUKA, ANTHONY JOSHUA GLENN, VILLAREAL VALERIE, GILBOA-GEFFEN ADI, STIDHAM SARAH, KEPLER JOHN H, MURPHY PATRICK
• Format: Patent
• Language: Chinese; English
• Published: 09.11.2018
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TESTING; VINEGAR; WINE

The present invention provides systems, devices, signaling polynucleotides (SPNs), detection agents and methods for detecting the presence and/or absence of one or more allergens in a sample particularly a food sample. The detection system includes a separate sampler, at least one disposable detection vessel for receiving and processing a test sample and a detection device for measuring a fluorescent signal. SPNs derived from aptamer that bind allergens are also provided as allergen detection agents. SPNs have a single open structure, and are probed with a fluorophore. Changes in fluorescencepolarization of detection agents upon the binding of allergens are measured to calculate the allergen content in a sample. 本发明提供用于检测样品特别是食物样品中种或多种变应原的存在和/或不存在的系统、装置、信号传导多核苷酸(SPN)、检测试剂和方法。检测系统包括单独的采样器，用于接收和处理测试样品的至少个次性检测容器和用于测量荧光信号的检测装置。来自结合变应原的适体的SPN作为检测试剂。SPN具有单的开放结构，并用荧光团标记。测量变应原结合时检测试剂的荧光偏振变化以计算样品中的变应原含量。