Rolling circle amplification primers, kit and method for quick detection of Staphylococcus aureus

• Main Authors: LI BING, LI LIN, XU ZHENBO, MIAO JIAN, SU JIANYU, XU XINGYONG, BAO XUERUI, LIU JUNYAN, LIU LIYAN
• Format: Patent
• Language: Chinese; English
• Published: 02.11.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses rolling circle amplification primers, kit and method for quick detection of Staphylococcus aureus. The primers include a padlock primer and RCA (rolling circle amplification) primers padF and padR, wherein the padlock primer has a nucleotide sequence shown as in SEQ ID NO. 1; the RCA detection primer padF has a nucleotide sequence shown as in SEQ ID NO. 2; the RCA detectionprimer padR has a nucleotide sequence shown as in SEQ ID NO. 3. By making use of the characteristic that a padlock probe can complementarily combine with template DNA, the padlock probe is designed on the specific gene fragment sequence of Staphylococcus aureus; rolling circle amplification is performed with primers; the rolling circle amplification detection method is established; the period oftraditional Staphylococcus aureus detection can be shortened; detection specificity and sensitivity are ensured. The rolling circle amplification primers, kit and method for quick detection of Staphylococcus aureus are of very