Antigen region for processing destroyed beta-conglycinin alpha'subunit based on phage showing technology positioning and screening method thereof

• Main Authors: LI SHUANG, XI JUN, LIU DEGUO, PI JIANGYI, CHEN ZHENNI
• Format: Patent
• Language: Chinese; English
• Published: 02.11.2018
• Subjects: CHEMISTRY; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES

The invention discloses an antigen region for processing destroyed beta-conglycinin alpha'subunit based on phage showing technology positioning and a screening method thereof. The amino acid sequenceof the antigen region is shown as SEQ ID NO.11. The antigen region for processing destroyed beta-conglycinin is researched by using a series of bioinformatics software, referring the beta-conglycininthree-dimensional crystalline structure analyzed by a PDB database and using a phage showing technology; the total length of the beta-conglycinin alpha'subunit and overlapping segmented protein are shown on the phage surface, so that regions with beta-conglycinin alpha'subunit antigenicity reduction caused by different processing methods are precisely positioned. Theoretical basis of processing method screening is provided for food industry; an application product for fast detecting the processed food desensitization effect can be further developed. 本发明公开了种基于噬菌体展示技术定位的加工破坏β-伴大豆球蛋白α'亚基的抗原区域及其筛选方法，所述抗原区域的氨基酸序列如SEQ ID NO.