Preparation and purification method and application of DAO protein

• Main Authors: ZHANG HAOTENG, WANG MAOFENG
• Format: Patent
• Language: Chinese; English
• Published: 26.10.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a preparation and purification method and application of DAO protein. The preparation and purification method comprises the following steps: step 1, preparing plasmids; step 2,expressing the protein; step 3, purifying the protein. The molecular weight of DAO-6H is 38kDa; according to Western blotting analysis and through detection by anti-His antibodies, the DAO-6H has a soluble portion that can be purified. The prepared DAO protein has great influence in schizophrenia, and preparation and purification of the DAO protein can be applied to a series of research on schizophrenia pathology, and are beneficial to deep study on mental diseases in modern medicine, thereby providing a method for treating the series of diseases. 本发明提出了种DAO蛋白的制备和纯化方法，包括以下步骤：步骤、质粒制备；步骤二、蛋白质的表达；步骤三、蛋白质的纯化。所述DAO-6H的分子量为38kDa，经由Western blotting分析，利用anti-His抗体侦测发现，DAO-6H具可溶部份可供纯化。本发明制备的DAO蛋白是精神分裂症中有重要影响的蛋白，其制备和纯化可用于精神分裂症病理的系列研究中，有助于现代医学对于精神病症的深入研究，对攻克该系列疾病提供了方法。