ENZYMATIC ACTIVITY OF LYTIC POLYSACCHARIDE MONOOXYGENASE

• Main Authors: FROMMHAGEN MATTHIAS, VISSER JACOB, KABEL MIRJAM ANNA, GRUPPEN HARM, HINZ SANDRA WILHELMINA AGNES, KOETSIER MARTIJN JOHANNES
• Format: Patent
• Language: Chinese; English
• Published: 23.10.2018
• Subjects: BAKERY PRODUCTS; BAKING; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; EDIBLE DOUGHS; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVATION THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS; VINEGAR; WINE

The present invention is in the area of enzymes for (hemi-)cellulose degradation and/or modification, more in particular the degradation and/or modification of xylan. The invention is based on a newlydiscovered enzymatic activity of a class of lytic polysaccharide monooxygenases (LPMOs), i.e.oxidative cleavage of xylan in addition to oxidative cleavage of cellulose. The present invention therefore relates to a method for degrading and/or modifying xylan in a xylan-comprising substrate, a method for preparing a product from a xylan-comprising substrate, a kit of parts, a liquid, paste or solidformulation, and a xylan-comprising composition, comprising said LPMO. The invention further relates to a use of said LPMO, said kit of parts, said formulation and/or said composition, in a method ofthe invention. 本发明处于用于(半)纤维素降解和/或改性的酶的领域，更具体是用于木聚糖的降解和/或改性的酶的领域。本发明基于类溶解性多糖单加氧酶(LPMO)的新发现的酶活性，即除了纤维素的氧化裂解之外还具有木聚糖的氧化裂解活性。因此，本发明涉及种用于对含木聚糖的底物中的木聚糖进行降解和/或改性的方法，种用于由含木聚糖的底物制备产物的方法，部件试剂盒，液体、糊剂或固体配制品，以及包含所述LPMO的含木聚糖的组合物。本发明进步涉及所述LP