EXONUCLEASE DEFICIENT POLYMERASES

• Main Authors: AYER ARUNA, DALAL DHRUTI, BIBILLO ARKADIUSZ, SARVABHOWMAN PREETHI, LEDERMAN ILYA, THAI EILEEN
• Format: Patent
• Language: Chinese; English
• Published: 23.10.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants havea strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase. 本文描述的是外切核酸酶缺陷的聚合酶变体。些变体保留与野生型或亲本聚合酶相当的链置换能力。些变体具有相对于野生型或亲本聚合酶改进的链置换能力。所述变体可以具有大于野生型或亲本聚合酶的延伸速率。所述变体可以具有小于野生型或亲本聚合酶的等待时间。