Preparation method of novel DNA (deoxyribonucleic acid) hydrogel

• Main Authors: FAN CHUNHAI, CHAO JIE, XING YIKANG, WANG LIANHUI
• Format: Patent
• Language: Chinese; English
• Published: 19.10.2018
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

The invention discloses a preparation method of a novel DNA (deoxyribonucleic acid) hydrogel. The preparation method comprises the following steps of S1, assembling single chains of DNA by self to form two module structures; S2, respectively performing agarose gel electrophoresis purifying and quantifying on the two module structures; S3, enabling initiating chains to trigger the two module structures to generate hybrid chain reaction, so as to form a DNA nanometer structure which is of a three-dimensional net shape; S4, placing the DNA nanometer structure of the three-dimensional net shape under an atom force microscope, imaging and observing; S5, hatching the established DNA nanometer structure of the three-dimensional net shape, so as to form the solid-state hydrogel. The preparation method has the advantages that the catalytic assembly process is a controllable process for establishing the hydrogel; in the reaction process, the initiating chains are used for triggering the two module structures to hybridi