INACTIVATION OF VIRUSES BY DELIPIDATION

• Main Authors: WU STEPHEN, DIAZ LEYLA
• Format: Patent
• Language: Chinese; English
• Published: 28.09.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention relates to vaccines containing enveloped viruses whose envelopes have been depleted of lipids using methyl [beta]-cyclodextrin (MBCD). Delipidation of enveloped viruses in a two-step process abolishes the infectivity of those viruses, which allows delipidated viruses to be used safely in vaccines. Use of MBCD to deplete lipids such as cholesterol, in contrast to other methods, results in viruses with less than 20% of the cholesterol of an untreated virus but such delipidated viruses retain at least 85% of the protein content of an untreated virus. Delipidation by MBCD also preserves the immunogenicity of the viral proteins. The present invention of using MBCD treatment in a two-step process to delipidate enveloped virus represents a new alternative for generation of inactivated viruses which are incorporated into effective vaccines. 本发明涉及含有包膜病毒的疫苗，所述病毒的包膜中已经使用甲基-β-环糊精(MBCD)将脂质消耗。以两步方法去脂包膜病毒消除了这些病毒的感染性，这使去脂病毒安全地用在疫苗中。与其他方法相反，使用MBCD消耗脂质如胆固醇导致病毒含有低于20％的未处理病毒胆固醇，但这种去脂病毒保留了至少85％的未处理病毒蛋白质含量。MB