In-vitro culture system for Tibetan yak oocyte

• Main Authors: WANG YUHENG, XU YEFEN, QIANGBAYANGZONG, SUOLANGSIZHU, NIU JIAQIANG
• Format: Patent
• Language: Chinese; English
• Published: 28.09.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to an in-vitro culture system for Tibetan yak oocyte. Researches show that in an in-vitro culture process of cumulus-oocyte complexes (COC) of yak, proliferation of granule cellsof the yak can be obviously improved by adding exogenous BMP15, and an effect of obviously improving the oocyte maturation rate is achieved. According to the culture system disclosed by the invention, the influence of the BMP15 on yak COC in-vitro culture and a molecular regulation mechanism thereof in an in-vitro maturation process can be explored, and a developmental mechanism of the yak oocytein the maturation process can be further known, so that the in-vitro maturation culture quality of the yak oocyte and the developmental capacity of early embryos can be improved. 本发明涉及种西藏牦牛卵母细胞体外培养体系。本发明研究发现，在牦牛卵丘卵母细胞复合体(cumulus-oocyte complexes,COC)体外培养过程中，添加外源BMP15能够显著提高牦牛颗粒细胞增殖，并且对卵母细胞成熟率有显著提高作用。本发明探索了体外成熟过程中BMP15对牦牛COC体外培养的影响及其分子调控机制，可以更进步了解成熟过程中牦牛卵母细胞的发育机制，以利于提高牦牛卵母细胞体外成熟培养的质量和早期胚胎的发育能力。