Detection method for diversity of microbial colony in brewing process of Baijiu
The invention relates to a detection method for the diversity of a microbial colony in the brewing process of Baijiu. The detection method comprises the following steps: firstly, pretreatment of a distiller's yeast sample: washing microbial strains in the distiller's yeast with normal sali...
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Main Authors | , , , , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
21.09.2018
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Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to a detection method for the diversity of a microbial colony in the brewing process of Baijiu. The detection method comprises the following steps: firstly, pretreatment of a distiller's yeast sample: washing microbial strains in the distiller's yeast with normal saline water and precipitating; secondly, DNA extraction: crushing the obtained microorganisms with liquid nitrogen, extracting with chloroform, ethanol and the like to obtain a DNA product and then dissolving with TEbuffer to obtain DNA dissolving liquid; thirdly, amplification of genomic DNA: taking total DNA of the sample as a template and adopting PCR to amplify; fourthly, gene sequencing: carrying out high-throughput sequencing on DNA amplification fragments by using a llumina Miseq sequencing platform;fifthly, data analysis: carrying out fusion processing on the obtained high-throughput sequencing data by using Flash software and comparing the data with an existing database to obtain strain information. Compared with the p |
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Bibliography: | Application Number: CN20181302155 |