Detection method for diversity of microbial colony in brewing process of Baijiu

• Main Authors: MENG WANGNI, DAI YIJIE, LIU TINGJU, CHEN MEI, HE HONG, XIAO LI, TIAN ZHIQIANG, YANG BO, SHU MEIBAO, SUN ZONGQI
• Format: Patent
• Language: Chinese; English
• Published: 21.09.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to a detection method for the diversity of a microbial colony in the brewing process of Baijiu. The detection method comprises the following steps: firstly, pretreatment of a distiller's yeast sample: washing microbial strains in the distiller's yeast with normal saline water and precipitating; secondly, DNA extraction: crushing the obtained microorganisms with liquid nitrogen, extracting with chloroform, ethanol and the like to obtain a DNA product and then dissolving with TEbuffer to obtain DNA dissolving liquid; thirdly, amplification of genomic DNA: taking total DNA of the sample as a template and adopting PCR to amplify; fourthly, gene sequencing: carrying out high-throughput sequencing on DNA amplification fragments by using a llumina Miseq sequencing platform;fifthly, data analysis: carrying out fusion processing on the obtained high-throughput sequencing data by using Flash software and comparing the data with an existing database to obtain strain information. Compared with the p