Preparation method of bacillus licheniformis with enhanced YvbW expression and obtained strain and application thereof

• Main Authors: ZHU JIANG, ZHU SHAN, LOU LIJUN, DUAN MAOHUA, LIU ZIWEI, LI JUNHUI, CHEN SHOUWEN, CAI DONGBO, CHEN XIAOBIN
• Format: Patent
• Language: Chinese; English
• Published: 21.09.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMES; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a preparation method of bacillus licheniformis with enhanced YvbW expression and an obtained strain and application thereof. By adopting a genetic engineering method, the bacillus licheniformis DW2-yvbW is obtained by inserting an amino acid permease YvbW gene into a bacillus licheniformis genome, and the yield of bacitracin in a fermentation liquor in the bacitracin fermentation process is improved by 13% or above by adopting the strain. 本发明提供种强化YvbW表达的地衣芽胞杆菌的制备方法和所得菌株及应用，本发明通过基因工程的方法通过在地衣芽孢杆菌的基因组内插入氨基酸通透酶YvbW基因，得到地衣芽孢杆菌DW2-，该菌株在杆菌肽发酵过程中发酵液中杆菌肽的产量提高了13%以上。