Engineering bacterium construction method fusing expression antibacterial peptide and red fluorescent protein

The invention discloses an engineering bacterium construction method fusing expression antibacterial peptide and red fluorescent protein. In a process of producing cationic antibacterial peptide LfcinB in genetic engineering, an mApple fusion partner gene is connected with a antibacterial peptide ge...

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Bibliographic Details
Main Authors GE MANMAN, ZHANG XURAN, ZHA XIANGDONG, RONG HUIJIE, YU ZHONGLI, YUN HUI
Format Patent
LanguageChinese
English
Published 11.09.2018
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses an engineering bacterium construction method fusing expression antibacterial peptide and red fluorescent protein. In a process of producing cationic antibacterial peptide LfcinB in genetic engineering, an mApple fusion partner gene is connected with a antibacterial peptide gene to construct a recombinant plasmid; a saccharomyces cerevisiae W303 competent cell is electrically transformed; adopted technologies are genetic engineering technologies including PCR (Polymerase Chain Reaction), restriction enzyme digestion, ligase connection and transformation and the like; thecationic antibacterial peptide is polypeptide with pure positive charge and antibacterial activity; an used vector plasmid is a plasmid pYES2-alpha carrying a saccharomyces cerevisiae alpha signal peptide gene. A saccharomyces cerevisiae engineering bacterium fuses the expression antibacterial peptide and the red fluorescent protein; the saccharomyces cerevisiae engineering bacterium has selective immune activation and r
Bibliography:Application Number: CN20181223031