RNA inhibitor and application thereof in inhibiting activity of F1F0-ATP synthase

• Main Authors: YAO KE, LIU SIYU, LUO YUEQIU
• Format: Patent
• Language: Chinese; English
• Published: 04.09.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses an RNA inhibitor and application thereof in inhibiting the activity of F1F0-ATP synthase. The RNA inhibitor has RNA sequences shown as SEQ ID NO.1-4. A small fragment RNA sequence is obtained through chemical synthesis, and the sequence can be combined with alpha subunits of the F1F0-ATP synthase in cells to release the alpha subunits of F1F0-ATP synthase from cell membranes to cytoplasm, so that F1F0-ATP synthase is depolymerized, and therefore intracellular ATP synthesis is inhibited. The RNA inhibitor has the advantages of high specificity and high inhibition efficiency. 本发明公开了种RNA抑制剂及其在抑制F1F0-ATP合成酶活性中的应用。该RNA抑制剂具有如SEQ ID NO.1～4所示的RNA序列。本发明通过化学合成的方法得到小片段的RNA序列，该序列能够结合细胞内的F1F0-ATP合成酶α亚基使F1F0-ATP合成酶的α亚基从胞膜释放到胞浆，导致F1F0-ATP合成酶解聚，从而抑制细胞内ATP合成，具有专性强，抑制效率高的特点。