In vitro fertilization culture solution and preparation method thereof

• Main Authors: LIU HONGJUN, YU YULU, YAN FEI, MI LE, DAI ZHEN
• Format: Patent
• Language: Chinese; English
• Published: 04.09.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the field of assisted reproduction and in particular relates to an in vitro fertilization culture solution and a preparation method thereof. A composition used for preparing the in vitro fertilization culture solution comprises resveratrol, reduced glutathione and lecithin, wherein a molar ratio of the resveratrol to the reduced glutathione to the lecithin is (5-50):(100-250):(8000-12000). The in vitro fertilization culture solution disclosed by the invention can achieve effects of providing a stable fertilization condition, removing reactive oxygen produced by cells and reactive oxygen free radicals produced by sperms in the motion process, reducing damage of the reactive oxygen to the sperms and ovum, improving the sperm motility, repairing cellular damage and improving the rate of fertilization and blastocyst formation rate, and the blastocyst formation rate is more than or equal to 90%. 本发明属于辅助生殖领域，具体涉及种体外受精培养液及其制备方法。用于制备体外受精培养液的组合物由白藜芦醇、还原型谷胱甘肽和卵磷脂组成；所述白藜芦醇、还原型谷胱甘肽和卵磷脂的摩尔比为5～50：1