Extraction and purification process for high-purity Chinese yam polysaccharides

• Main Authors: LIU JINGBIN, ZHU YONGNING, XIE YINZHI, ZHU XUELAN
• Format: Patent
• Language: Chinese; English
• Published: 28.08.2018
• Subjects: CHEMISTRY; COMPOSITIONS BASED THEREON; DERIVATIVES THEREOF; METALLURGY; ORGANIC MACROMOLECULAR COMPOUNDS; POLYSACCHARIDES; THEIR PREPARATION OR CHEMICAL WORKING-UP

The invention provides an extraction and purification process for high-purity Chinese yam polysaccharides. The extraction and purification process comprises the following steps: firstly, peeling and slicing fresh Chinese yam, adding the sliced fresh Chinese yam into an aqueous solution containing 0.5% of citric acid, 0.4% of sodium citrate and 0.2% of ascorbic acid, carrying out grinding with a burrstone mill, then carrying out separation with a centrifuge three times to remove fibers and black residues, adding immobilized composite protease into a filtrate, and carrying out stirring and enzymatic hydrolysis under heat preservation conditions; and secondly, separating proteins and polysaccharides in the Chinese yam, carrying out hydrolyzing to obtain amino acids and peptide fragments, filtering out the immobilized composite protease, carrying out separating with a decanter centrifuge to remove starch and obtain clear liquid, filtering the clear liquid with a membrane, collecting a filtrate, concentrating the