Rhinogobio ventralis Sauvage et Da bry paternity testing method based on micro-satellite fluorescence multiplex PCR

• Main Authors: YANG DEGUO, WU XINGBING, CHEN LIANG, ZHU YONGJIU, HE YONGFENG
• Format: Patent
• Language: Chinese; English
• Published: 17.08.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a Rhinogobio ventralis Sauvage et Da bry paternity testing method based on micro-satellite fluorescence multiplex PCR. The Rhinogobio ventralis Sauvage et Da bry paternity testing method comprises: 1, extracting the genomic DNA of a to-be-tested sample; 2, carrying out a multiplex PCR amplification reaction by using a primer combination, and carrying out allele typing by using a sequencer; and 3, detecting the correlation between a to-be-tested subject and a parental genotype through likelihood ratio, and determining the paternity. Compared to the traditional PCR method, the method of the present invention has the following advantages that the detected microsatellite loci are increased by about 3 times so as to substantially improve the efficiency and the speed of the paternity testing, the detection cost is only about 1/3 of the original detection cost, the allele size interpretation error and other problems in the typing using the acrylamide gel electrophoresis are overcome, the accu