Long-term genome DNA preservation method and kit

• Main Authors: CUI LIANGHONG, LIU BING, CHEN LANQUN, CHANG XIAOYONG, LIU ZHI, ZHU QIANQIAN, XIA ZHENGXIANG, LU DEQUAN
• Format: Patent
• Language: Chinese; English
• Published: 17.08.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a long-term genome DNA preservation method and kit and particularly provides a genome DNA long-term preservation method, comprising the steps of adsorbing a target gene via a solid carrier, washing to remove impurities, and drying to remove liquid components so as to provide long-term preservation for genome DNA. The long-term expected valid preservation time of genome DNA preserved via method may reach hundred years. Genome total DNA is adsorb-preserved via a solid phase material according to the method herein; the integrity, physio-chemical properties and biological features of genome DNA structure are never affected, genetic diversity of the target gene can be maintained, and the expected valid preservation time may reach hundred gears and longer. 本发明提供了长期基因组DNA的保存方法及试剂盒，具体地本发明提供的基因组DNA的长期保存方法包括步骤：固相载体吸附目的基因，洗涤去除杂质，干燥去除液体成分，从而实现对基因组DNA的长期保存。使用本发明的方法保存基因组DNA，保存方法的长期预期有效保存时间可达百年。根据本发明的方法使用固相材料吸附保存基因组总DNA，不会影响基因组DNA结构的完整性、理化性质、及其生物特性，而且可以保持目的基因的遗传多样性，预期有效保存时间可达百年以上。