Serum-free culture medium for adherent cell culture and application of serum-free culture medium

• Main Authors: GONG LINGLI, HU YALING, JI LI, ZOU JIAN, WANG JINGJING, ZOU YISU, YIN YING
• Format: Patent
• Language: Chinese; English
• Published: 17.08.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a serum-free culture medium for adherent cell culture and application of the serum-free culture medium, and belongs to the field of cell culture media. High-glucose DMEM (dulbecco's modified eagle media) and serum-reduced culture media are used as basic culture media, and the basic culture media contain serum substitutes, compound amino acid, penicillin and streptomycin. Theserum-free culture medium and the application have the advantages that the serum-free culture medium is free of cell culture media with animal-derived substances and accordingly is independent from animal serum; the serum-free culture medium comprises definite components, an alternative scheme for serum culture media is provided, and accordingly the serum-free culture medium is favorable for market promotion; the serum-free culture medium can be applied to the adherent cell culture; self-renewal of tumor cells and normal cell lines which are subjected to adherent culture can be maintained bythe serum-free culture med