Serum-free culture medium for adherent cell culture and application of serum-free culture medium
The invention provides a serum-free culture medium for adherent cell culture and application of the serum-free culture medium, and belongs to the field of cell culture media. High-glucose DMEM (dulbecco's modified eagle media) and serum-reduced culture media are used as basic culture media, and...
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Main Authors | , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
17.08.2018
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Subjects | |
Online Access | Get full text |
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Summary: | The invention provides a serum-free culture medium for adherent cell culture and application of the serum-free culture medium, and belongs to the field of cell culture media. High-glucose DMEM (dulbecco's modified eagle media) and serum-reduced culture media are used as basic culture media, and the basic culture media contain serum substitutes, compound amino acid, penicillin and streptomycin. Theserum-free culture medium and the application have the advantages that the serum-free culture medium is free of cell culture media with animal-derived substances and accordingly is independent from animal serum; the serum-free culture medium comprises definite components, an alternative scheme for serum culture media is provided, and accordingly the serum-free culture medium is favorable for market promotion; the serum-free culture medium can be applied to the adherent cell culture; self-renewal of tumor cells and normal cell lines which are subjected to adherent culture can be maintained bythe serum-free culture med |
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Bibliography: | Application Number: CN20181211964 |