Method for determining free polysaccharide in streptococcus pneumoniae capsular polysaccharide conjugate

• Main Authors: WANG HAIQI, LI QING, LI HUIYUN, YANG YAN, XU LAIFANG, AN WENQI
• Format: Patent
• Language: Chinese; English
• Published: 10.08.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a method for determining the content of free polysaccharide in a streptococcus pneumoniae capsular polysaccharide conjugate. The scheme comprises: adding a sodium deoxycholate solution to streptococcus pneumoniae capsular polysaccharide and a conjugate corresponding to the streptococcus pneumoniae capsular polysaccharide, adjusting the pH to 2-6, and performing centrifugation to collect the supernatant; adding a color reagent to the streptococcus pneumoniae capsular polysaccharide supernatant to carry out a color development reaction; adding a color reagent to the supernatant of the conjugate corresponding to the streptococcus pneumoniae capsular polysaccharide to carry out a color development reaction; and determining the absorbance and calculating the content of free polysaccharide. The method for quantitative determination of free polysaccharide in a streptococcus pneumoniae capsular polysaccharide conjugate is low in analytical cost and simple to operate. 本发明提供了种检测肺炎链球菌荚膜多糖结合物中游离多糖含