Trace silk fibroin enrichment method

• Main Authors: MA CHAO, ZHENG HAILING, DAI XIANJUN, ZHOU YANG
• Format: Patent
• Language: Chinese; English
• Published: 10.08.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a trace silk fibroin enrichment method. The method comprises the following steps: 1) mixing of different silk fibroin monoclonal antibodies; 2) a coupling reaction; 3) obtaining of immunomagnetic beads; 4) identification; 5) silk fibroin monoclonal antibody immobilization of the coupled immunomagnetic beads; 6) binding of the silk fibroin monoclonal antibody immunomagneticbeads to silk fibroins; 7) elution of the silk fibroins; 8) detection; 9) standard adding recovery. The immunomagnetic beads are prepared from the composite monoclonal antibodies of the silk fibroins,and the immunomagnetic beads are used to enrich the trace silk fibroins, so the trace silk fibroins in an archaeological soil sample can be well extracted, and the method has the advantages of extraction of different decomposition fragments, high enrichment efficiency, simplicity in operation, and short experiment time. 本发明公开了种痕量丝素蛋白富集方法，包括以下步骤：1）不同丝素蛋白单克隆抗体的混合；2）耦联反应；3）免疫磁珠的获得；4）鉴定；5）耦联后免疫磁珠的丝素蛋白单克隆抗体固定化；6）丝素蛋白单克隆抗体免疫磁珠与