SSR (simple sequence repeat) primer and method for identifying purity of watermelon hybrid seeds Anran and yellow phoenix

• Main Authors: LU XIA, DENG ZHIJUN, SI LONGTING, TENG LUHUA, A MEN
• Format: Patent
• Language: Chinese; English
• Published: 10.08.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an SSR (simple sequence repeat) primer for identifying the purity of watermelon hybrid seeds Anran and yellow phoenix. The primer comprises an upstream primer CISSR18153-F anda downstream primer CISSR18153-R of a primer CISSR18153, the nucleotide sequence of the upstream primer CISSR18153-F is as shown in SEQ ID NO. 1, and the nucleotide sequence of the downstream primer CISSR18153-R is as shown in SEQ ID NO. 2. PCR (polymerase chain reaction) amplification is performed by the primer CISSR18153, 142bp of male parent specific markers and 152bp of female parent specificmarkers can be amplified in the watermelon hybrid seeds Anran, and 152bp of male parent specific markers and 142bp of female parent specific markers can be amplified in the watermelon hybrid seeds yellow phoenix. By the aid of the primer CISSR09643, the hybrid seeds of watermelon varieties Anran and yellow phoenix can be distinguished from corresponding male parent seeds and female parent seeds and other accessory seeds, a