EVALUATION METHOD FOR DIFFERENTIATION STATE OF CELLS

• Main Authors: HIRAMARU DAISUKE, TOMITA KENTARO, OZAKI SHIGENORI, TAKAHASHI MASATOSHI, HATABAYASHI KUNITADA, KAGAWA KENICHI, SUZUKI TAKASHI
• Format: Patent
• Language: Chinese; English
• Published: 31.07.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention is a method for evaluating the differentiation state of test cells on the basis of the amount of a prescribed indicator substance that is present on a culture supernatant for thetest cells, which are stem cells that have an unknown differentiation state or cells that have been induced to differentiate from stem cells. The indicator substance is at least one compound selectedfrom the group that consists of ornithine, 2-aminoadipic acid, deoxycytidine, glutamic acid, tryptophan, asparagine, alanine, cystine, hypoxanthine, uridine, aspartic acid, arginine, 2-hydroxybutyricacid, 2-hydroxyisovalerate, 3-hydroxyisovalerate, urea, 4-hydroxybenzoic acid, 4-aminobenzoic acid, ribonic acid, kynurenine, cystathionine, threonic acid, pyruvic acid, putrescine, ascorbic acid, riboflavin, serine, cysteine, orotic acid, and citric acid. According to one embodiment, differentiation state is evaluated on the basis of the amount of two or more indicator substances that is present. 本发明是种细胞分化状态的评价方法，将分化状态未知的