Modified anthrax toxin protective antigen

• Main Authors: WEIN ALEXANDER N, LEPPLA STEPHEN H, BUGGE THOMAS H, LIU JIE, PETERS DIANE E, LIU SHI-HUI, CHEN KUANG-HUA
• Format: Patent
• Language: Chinese; English
• Published: 31.07.2018
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

Disclosed is a Bacillus anthracis protective antigen (PA) comprising a PA amino acid sequence, wherein one or more of amino acid residues I207, I210, E654, I656, R659, M662, Y681, and L687, as definedby reference to SEQ ID NO: 1, are, independently, substituted, with the proviso that amino acid residue I207 is not substituted with alanine and amino acid residue I210 is not substituted with alanine. Related compositions, nucleic acids, recombinant expression vectors, host cells, populations of cells, methods of treating or preventing cancer in a mammal, and methods of inhibiting the growth ofa target cell are also disclosed. 公开了包含保护性抗原(PA)氨基酸序列的炭疽芽孢杆菌(Bacillus anthracis)PA，其中参照SEQ ID NO:1所定义的氨基酸残基I207、I210、E654、I656、R659、M662、Y681和L687中的个或多个被独立地取代，条件是氨基酸残基I207不被丙氨酸取代，并且氨基酸残基I210不被丙氨酸取代。还公开了相关组合物、核酸、重组表达载体、宿主细胞、细胞群、治疗或预防哺乳动物的癌症的方法以及抑制靶细胞生长的方法。