Biomass-spectrometry relative quantification method for DNA methylase subtypes of zebrafish

• Main Authors: ZENG YANYAN, ZHU DAN, FAN HONGBO, YUAN MEIYI, LIN BOJI, HU JUNJIE, LAO ZHILANG, LIANG ZHIHUI, LI YANLIANG, WU KANGMING
• Format: Patent
• Language: Chinese; English
• Published: 27.07.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a biomass-spectrometry relative quantification method for the DNA methylase subtypes of zebrafish. Seven subtypes of the DNA methylase of zebrafish are involved in the invention and comprise DNMT1, DNMT3, DNMT4, DNMT5, DNMT6, DNMT7 and DNMT8. The method comprises the following steps: step 1, pretreatment of protein samples; step 2, preparation of a standard curve; and step3, determination of the content of the DNMT subtypes in the protein samples. The method provided by the invention adopts a plurality of specific peptide fragments for analysis and quantification of each DNMT subtype simultaneously, eliminates errors brought by sample treatment and determination and is improved in accuracy. 本发明涉及种斑马鱼DNA甲基化酶亚型质谱相对定量方法，涉及斑马鱼的DNA甲基化酶的7种亚型，包括DNMT1、DNMT3、DNMT4、DNMT5、DNMT6、DNMT7以及DNMT8，包括以下步骤如下：步骤1、蛋白样品预处理；步骤2、标准曲线制备；步骤3、蛋白样品中DNMT亚型含量测定。本发明相方法对每种DNMT亚型利用多种特异性肽段同时进行分析和定量，消除样品处理及测定所带来的误差，提高方法准确度。