Mitochondrial gene detection kit and use method thereof

• Main Authors: ZHANG HUALIN, QI YU, WU FENG, LIAO YONG, YUAN YANJIAO, WAN HUAJING, LIU HONGWEI
• Format: Patent
• Language: Chinese; English
• Published: 24.07.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention provides a mitochondrial gene detection kit and a use method thereof. The mitochondrial gene detection kit comprises two groups of primer pairs, a reagent for PCR amplification and a reagent for sanger sequencing. The mitochondrial gene detection kit provided by the invention has characteristics of being low in detection cost, convenient to operate, high in sensitivity, high in specificity and the like, and can be widely applied to individual identification, genetic relationship identification and other forensic medicine fields. 本发明提供种线粒体基因检测试剂盒及其使用方法，该线粒体基因检测试剂盒包括两组引物对、用于PCR扩增的试剂，以及用于sanger测序的试剂。本发明提供的线粒体基因检测试剂盒具有检测成本低、操作方便、灵敏度高、特异性高等特点，能广泛应用于个体识别、亲缘关系鉴定等法医学领域。