PLANT EPSP SYNTHASES AND METHODS OF USE

• Main Authors: DONG YUXIA, NG EMILY, PATTEN PHILLIP A, SIEHL DANIEL, LU JIAN, LASSNER MICHAEL
• Format: Patent
• Language: Chinese; English
• Published: 17.07.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Compositions and methods comprising polynucleotides and polypeptides having EPSP (5-enolpyruvylshikimate-3-phosphate) synthase (EPSPS) activity are provided. In specific embodiments, the sequence hasan improved property, such as, but not limited to, improved catalytic capacity in the presence of the inhibitor, glyphosate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the EPSPS sequences. Various methods of employing the EPSPS sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explantor seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein. 提供了包含多核苷酸和具有EPSP(5-烯醇丙酮莽草酸-3-磷酸)合酶(EPSPS)活性的多肽的组合物和方法。在具体实施例中，序列具有改进的特性，例如但不限于在抑制剂草甘膦的存在下，改进的催化能力。进步提供了具有EPSPS序列的核酸构建体、植物、植物细胞、外植体、种子和籽粒。提供了采用EPSPS序列的不同方法。此类方法包括用于产生草甘膦耐受性植物、植物细胞、外植体或种子的方法，以及在含有采用本文公开的植物和/或种子的作物的田地中，控制杂草的方法。