Single particle biological probe and construction method for plasma biological storage device thereof

• Main Authors: ZHANG YING, SHUAI ZHENHUA, ZHANG LEI, WANG LIANHUI, ZHOU HAO, WENG LIXING
• Format: Patent
• Language: Chinese; English
• Published: 17.07.2018
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a single particle biological probe and a construction method for a plasma biological storage device thereof. The construction method comprises the following steps: preparing a gold and silver core-shell nanometer cube (Au@AgNC2) sol and a tsDNA solution respectively, and fixing the Au@AgNC2 to the surface of indium tin oxide conducting film glass; dropwise adding the tsDNA solution to the surface of the indium tin oxide conducting film glass fixed with the Au@AgNCs, after incubation at room temperature, washing with ultrapure water, drying with nitgrogen, and thus obtaining the single particle LSPR biological probe based on tsDNA. Compared with a nanometer cube, the Au@AgNCs has similar plasma characteristics and more stable structure; and compared with a single-chain DNA molecule of a one-dimensional structure and a hairpin type DNA molecule of a two-dimensional structure, the tsDNA of a three-dimensional structure has the advantages of higher rigidity, structure stability, easy multi-