GENETIC ENGINEERING OF MACROPHAGES FOR IMMUNOTHERAPY

• Main Authors: CRANE COURTNEY, JENSEN MICHAEL C, PERRY NICOLE, WHITE MOYES KARA
• Format: Patent
• Language: Chinese; English
• Published: 03.07.2018
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

Disclosed are methods of making a genetically modified immune cell for modifying a tumor microenvironment (TME) and methods of modifying a tumor microenvironment (TME). In some embodiments, the methodcan include delivering a first vector to an immune cell, wherein the first vector comprises a nucleic acid encoding a protein that induces T-cell proliferation, promotes persistence and activation ofendogenous or adoptively transferred NK or T cells and/or induces production of an interleukin, an interferon, a PD-1 checkpoint binding protein, HMGB1, MyD88, a cytokine or a chemokine. Methods of modulating the suppression of the immune response in a tumor microenvironment, minimizing the proliferation of tumor and suppressive cells, and increasing the efficiency of an anti-cancer therapy, anti-infection therapy, antibacterial therapy, anti-viral therapy, or anti-tumoral therapy are also provided. 公开了制造用于修饰肿瘤微环境(TME)的遗传修饰的免疫细胞的方法和对肿瘤微环境(TME)进行修饰的方法。在些实施方式中，该方法可包括将第载体递送至免疫细胞，其中，所述第载体包含编码蛋白的核酸，所述蛋白诱导T细胞增殖；促进内源性或过继性转移的